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| Registros recuperados: 14 | |
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| Alves,L.P.S.; Almeida,A.T.; Cruz,L.M.; Pedrosa,F.O.; de Souza,E.M.; Chubatsu,L.S.; Müller-Santos,M.; Valdameri,G.. |
| The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flow cytometry; Nile red; Poly-3-hydroxybutyrate; Herbaspirillum seropedicae; Azospirillum brasilense. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100606 |
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| Guimarães,Salomão Lima; Baldani,José Ivo; Baldani,Vera Lúcia Divan; Jacob-Neto,Jorge. |
| O objetivo deste trabalho foi avaliar o efeito da utilização de bactérias diazotróficas com ou sem molibdênio em duas cultivares de arroz com diferentes potenciais de fixação biológica de nitrogênio. Dois experimentos foram montados: o primeiro foi conduzido em condições gnotobióticas, com utilização das estirpes BR 11417 (ZAE94 - Herbaspirillum seropedicae) e BR 11340 (M130 - Burkholderia sp.), duas doses de molibdênio, 1,12 e 2,25 g, e duas fontes, molibdato de sódio e molibdato de amônio. As sementes foram peletizadas com inoculante turfoso com bactérias diazotróficas e o molibdênio. Em seguida as cultivares foram plantadas em tubos de ensaio com capacidade para 120 mL com 60 mL da solução de Hoagland sem nitrogênio por 30 dias. Foi avaliada a matéria... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oriza sativa; Herbaspirillum seropedicae; Burkholderia; Molibdato de sódio; Molibdato de amônio. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300013 |
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| Monteiro,Rose A.; Schmidt,Maria A.; Baura,Valter A. de; Balsanelli,Eduardo; Wassem,Roseli; Yates,Marshall G.; Randi,Marco A.F.; Pedrosa,Fábio O.; Souza,Emanuel M. de. |
| The bacterium Herbaspirillum seropedicae is an endophytic diazotroph found in several plants, including economically important poaceous species. However, the mechanisms involved in the interaction between H. seropedicae and these plants are not completely characterized. We investigated the attachment of Herbaspirillum to maize roots and the invasion of the roots by this bacterium using H. seropedicae strain SMR1 transformed with the suicide plasmid pUTKandsRed, which carries a mini-Tn5 transposon containing the gene for the Discosoma red fluorescent protein (Dsred) constitutively expressed together with the kanamycin resistance gene. Integration of the mini-Tn5 into the bacterial chromosome yielded the mutant H. seropedicae strain RAM4 which was capable of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Confocal microscopy; Diazotroph; Endophytic; Herbaspirillum seropedicae. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500021 |
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| Aquino,B.; Stefanello,A.A.; Oliveira,M.A.S.; Pedrosa,F.O.; Souza,E.M.; Monteiro,R.A.; Chubatsu,L.S.. |
| NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological nitrogen fixation; Herbaspirillum seropedicae; NifA. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800683 |
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| Schmidt,M.A.; Souza,E.M.; Baura,V.; Wassem,R.; Yates,M.G.; Pedrosa,F.O.; Monteiro,R.A.. |
| Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbaspirillum seropedicae; Phaseolus vulgaris; Confocal microscopy. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300001 |
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| Souza,André L.F.; Chubatsu,Leda S.; Souza,Emanuel M.; Pedrosa,Fábio O.; Monteiro,Rose A.; Rego,Fabiane G.M.; Rigo,Liu U.. |
| In prokaryotes molybdenum is taken up by a high-affinity ABC-type transporter system encoded by the modABC genes. The endophyte β-Proteobacterium Herbaspirillum seropedicae has two modABC gene clusters and two genes encoding putative Mo-dependent regulator proteins (ModE1 and ModE2). Analysis of the amino acid sequence of the ModE1 protein of H. seropedicae revealed the presence of an N-terminal domain containing a DNA-binding helix-turn-helix motif (HTH) and a C-terminal domain with a molybdate-binding motif. The second putative regulator protein, ModE2, contains only the helix-turn-helix motif, similar to that observed in some sequenced genomes. We cloned the modE1 (810 bp) and modE2 (372 bp) genes and expressed them in Escherichia coli as His-tagged... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbaspirillum seropedicae; ModE1 protein; ModE2 protein. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400022 |
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| Sousa, Ricardo Felipe Braga; Guimarães, Vandeir Francisco; Pinto Jr., Artur Soares; Costa, Andréia Cristina Peres Rodrigues; Offemann, Luiz Claudio; Fuchs, Felipe; Coelho, Gustavo Ferreira. |
| This work aimed to evaluate physiological characteristics of corn seeds provenient from cultivation associated with Azospirillum brasilense and Herbaspirillum seropedicae and reinoculated with Azospirillum brasilense. The seeds were coming from cultivation in field conditions, and the treatments were: T1 - control; T2 - 60 kg hectare-1 N, T3 - inoculation (Azospirillum brasilense - AbV5); T4 - inoculation (Azospirillum brasilense) + 60 kg hectare-1 N; T5 - inoculation (Herbaspirillum seropedicae - SMR1) and T6 - inoculation (Herbaspirillum seropedicae) + 60 kg hectare-1 N. The treatments were divided in two separate blocks, being one reinoculated with A. brasilense (AbV5). The experimental design was completely randomized, in factorial scheme (6x2), being... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azospirillum brasilense; Herbaspirillum seropedicae; Seeds physiology; Reinoculation; Zea mays. |
| Ano: 2015 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/8753 |
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| Marques Júnior,Roberto Batista; Canellas,Luciano Pasqualoto; Silva,Lúcia Gracinda da; Olivares,Fábio Lopes. |
| Além do fornecimento direto de nutrientes por meio da mineralização da matéria orgânica ou pela fixação biológica de N2, as substâncias húmicas e as bactérias diazotróficas endofíticas podem afetar diretamente o metabolismo vegetal, modificando o padrão de crescimento e desenvolvimento das plantas. O objetivo deste trabalho foi avaliar o efeito do uso em conjunto de ácidos húmicos (AH) e Herbaspirilum seropedicae, uma bactéria endofítica fixadora de N2, sobre o crescimento radicular de microtoletes de cana-de-açúcar do cultivar RB 72 454 tratados termicamente ou não. Após o tratamento térmico, os microtoletes foram imersos por 12 h em água (controle), numa solução que continha 20 mg L-1 de C de AH isolados de vermicomposto, H. seropedicae estirpe HRC 54 na... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbaspirillum seropedicae; Termoterapia; Ácidos húmicos. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000300020 |
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| Galvão,C.W.; Souza,E.M.; Etto,R.M.; Pedrosa,F.O.; Chubatsu,L.S.; Yates,M.G.; Schumacher,J.; Buck,M.; Steffens,M.B.R.. |
| DNA repair is crucial to the survival of all organisms. The bacterial RecA protein is a central component in the SOS response and in recombinational and SOS DNA repairs. The RecX protein has been characterized as a negative modulator of RecA activity in many bacteria. The recA and recX genes of Herbaspirillum seropedicae constitute a single operon, and evidence suggests that RecX participates in SOS repair. In the present study, we show that the H. seropedicae RecX protein (RecX Hs) can interact with the H. seropedicaeRecA protein (RecA Hs) and that RecA Hs possesses ATP binding, ATP hydrolyzing and DNA strand exchange activities. RecX Hs inhibited 90% of the RecA Hs DNA strand exchange activity even when present in a 50-fold lower molar concentration than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RecA; RecX; Herbaspirillum seropedicae; SOS repair. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200004 |
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| Registros recuperados: 14 | |
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